Teaching a Beginner How to Make Tissue Culture Media
Teaching a Beginner How to Make Tissue Culture Media
Our lab director, Francisco, teaches our Digital Marketing Manager (a plant tissue culture beginner), Rachel, how to make liquid tissue culture media step by step. Watch now to follow along and learn how to make it yourself! Get 10%OFF the tissue culture products you need to follow along: https://www.plantcelltechnology.com/?ref=FP10
We are Plant Cell Technology, a leading plant tissue culture industry organization since 1995. We develop, produce, and market innovative solutions for global plant tissue culture operations and hobbyists. Our well-researched, certified, and trusted products keep your cultures healthy and contaminate-free.
Follow us on social media!
INSTAGRAM: https://www.instagram.com/plant.cell.technology/
FACEBOOK: https://www.facebook.com/plantcelltechnology
LINKEDIN: https://www.linkedin.com/company/plant-cell-technology-inc-/
TIKTOK: https://www.tiktok.com/@plantcelltech
TWITTER: https://twittter.com/plantcellnews
rare house plants, tissue culture, master class, carnivorous plants, plant propagation, sustainable, growth, care tips, update, houseplant trends
#houseplants #rareplants #tissueculture #masterclass #carnivorousplants #propagation #sustainable #update #plantcare #plantgrowth
#PlantCellTechnology #TissueCultureMasterClass #TissueCulture #MicroPropagation #Plants #Labratory #Lab #TCLab #LiquidMediaCultures #MicroBiology #Cultures #LiquidMedia #TC #planttest #tissueculturetest #protocols #tissuecultureprotocols #coffeechat #tissueculturequestions #tissuecultureanswers #planttissueculture #plants #plant #propagation #learntissueculture #tissuecultureclass #tissuecultureeducation
#PlantCellTechnology #TissueCulture #Propagation #MicroPropagation #Plants #Labratory #Lab #TCLab #LabEquipment #LiquidMediaCultures #MicroBiology #Cultures #LiquidMedia #TC
#PlantCellTechnology #TissueCulture #Propagation #MicroPropagation #Plants #Labratory #Lab #TCLab #LiquidMediaCultures #MicroBiology #Cultures #LiquidMedia #TC #planttest #tissueculturetest #protocols #tissuecultureprotocols #coffeechat #tissueculturequestions #tissuecultureanswers #planttissueculture #plants #plant #tissueculturemasterclass #biocoupler #temporaryimmersion #liquidmedia
Can you teach me the protocol for date palm please thank you
How come you did not use agar?
what if i add agfar and sset ph after that?
Hello Fransisco , great videos im consuming them all since several Days.
For me as a wannabe hobby wanna be Tissue culture starter i still got several questionmarks.
One of them is , what plant material excatly do i add and when can i multiply these , because for me it just sound like you put a node wait till new grow and take that new node , which makes 2-3 Plants.
But obviously thats not the quantity people are Producing with Tissue culture so how do they do it ?
And also there must be some kind of gathering for atleast some recipes right ?
Do you guys have a video or a how to on your side that im missing ?
Would be awesome if you could answer my first question in particular.
Thank you for your time.
Taximo add panalama
Ok i learned some new stuff as a complete newbie but the the last ingredients wasnt clear and some links doesn’t work
Can we get more of these? Theyre super informative, maybe one on sterilisation and placing the plants in the media. Very helpful for beginners 😊
Cool video! Here are my notes:
General Multiplication Tissue Culture Medium:
To 800 ml of Distilled water add:
30 grams Sucrose Sugar (for Carbohydrates)
4.54 grams of MS Salts Media (for Micro and Macronutrients)
Using a Micropipettor add:
1 ml PPM Plant Preservative Mixture (Biocide to prevent contaminants)
Plant Growth Regulators:
1 ml Benzylaminopurine/BA/BAP (cytokinin for shoot initiation)
0.1 ml Naphthaleneacetic Acid / NAA (rooting agent)
Then add distilled water to bring total volume to 1L
Calibrate the pH to 5.8: (Store your probes in 3 Mol KOH, rinse in distilled water)
To go Higher (more basic), use Sodium Hydroxide
To go Lower (more acidic), use Hydrochloric Acid
Optional; Add gel medium: (more gel medium makes a more firm medium)
If using Agar – 7-10 grams depending on firmness desired
If using gellan gum 2-4 grams depending on firmness desired
500 ml Half Batch Calculator:
Liters of Water: 0.5
Agar (g): 3.5
MS Media (g): 2.27
PPM (ml): 0.5
Should not we sterilize all the equipment before using preparing the medium????
In all of your bio-coupler videos, it appears that you are fully sealing the bio-couplers before autoclaving them. Are you loosely connecting them to avoid pressure and breaking in the autoclave?
Can you make a video for macroalgae propagation through semi liquid media…
I actually want video Tortorial for it using PES media
My question is wy did you gave me a none existing address to buy your tissue culture mediums in Johannesburg South Africa
In my experience, the PH prior to autoclave and PH after autoclave varies slightly, and the final PH Pre-inserting the plant and post removing the plant (eg. measurement of leftover media after two months of plant growth).
I get if the PH is too high or too low, it would be detrimental, and even affect gelling ability if swung too high or too low, but in general if it’s within 4.5-7 pre autoclave, is PH adjustment really necessary?
Thanks for your insight.
What could you use if you dont have a autoclave?