Can Tissue Culture Remove Hop Latent Viroid (HLVd)
Can Tissue Culture Remove Hop Latent Viroid (HLVd)
Can Tissue Culture Remove Hop Latent Viroid (HLVd)?
Professor DeBacco
Testing/Screening is Still Needed
This is still an important part of the process.
By testing plants that are infected can be culled out as even tissue culture does not ensure a clean plant.
However, culturing from a clean plant and producing known clean plants is a method that can lead to a “clean” facility.
*Testing/screening should still occur, if you have employees, bring other plant material in, plan on keeping a plant as a mother and to know you do (still) have a clean facility.
Is Tissue Culture the Answer?
Based on hops it is possible to get clean tissue and eliminate the passage of the viroid to the new tissue.
Portion of the plant the sample is taken and cultivar being used for the plant cells all have an impact on the effectiveness of this process.
However, when multiple rounds are done with testing (screening) in-between it is possible to rid a line of the viroid.
Meristem Culture preferred
With tissue culture you only need a small number of initial cells, so where should you take them from?
Meristem culture is more advantageous compared to using material from a node.
Remember that the viral load can vary in a plant
Some people think this is because the plant grows quicker than the viroid can spread and this may be true to an extent, but in reality the viroid is not far away.
Common Locations based on Tomatoes
When inoculated it is more likely to be found above (rather than below) the point of intentional infection
(At least initially)
It is very common in roots and this may be where it initially concentrates.
Look to the Potato
Potatoes are vegetatively propagated
These are often grown in tissue culture to produce virus free plant stock that will be used for a number of years and then the tissue culture process is repeated to help ensure clean tissue.
Why Meristem Regions?
The very tip of the plant is the new fastest growing cells that may be able to out pace the growth/spread of the virus.
This is not a guarantee, but it does help potentially increase the odds of generating virod free tissue.
Link to Lecture Slides: https://drive.google.com/file/d/1nAJPbsZHfFOPyTZh40E8JfoDsf45kqSH/view?usp=drive_link
*Due to the description character limit the full work cited for “Can Tissue Culture Remove Hop Latent Viroid (HLVd)” can be viewed at… https://docs.google.com/document/d/15AiQ0J99KVdT-ybGYf7x8467NEZJ0YeC/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true
Like always thanks for your information and great videos !
Your subject matter is never boring or redundant and quite easily understood.
Thanks for making us all better growers by gaining knowledge.
Hello friends, I am suffering from wilting trees in the field. Is there a solution for that?
Valuable info. Thanks
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Thanking you very kindly for keeping us updated on this matter we are very grateful for all the information you have provided us regarding this issue. This actually looks like a glimpse of light it what was starting out to be a absolute nightmare for a wonderful plant family. I can’t thank you enough for the kind heartfelt advice you are providing us with on this subject. Have a beautiful day and more importantly stay safe and healthy my friend 🫵🏻😘👍🏼
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Hi, if I have 200ml of 3% hydrogen peroxide, how much distilled water would I need for the magic 1 %? Is there a formula for thick people that are down to ten 9 year old seeds and in China and can not get anymore! Also, they are all auto if I can get just one to germinate any advise on how to stress the plant to become a hermi? Sorry for asking this here. Any advice would be appreciated. When I came here ten years ago, seeds, growing technically wegal. Then CBD came flooding in and the gov knee jerked so now even eeds can land you five years in P. This is YT jalking in wode is now necessary.
Could you do a video on electroculture
Nice to see you are purtting out more vids
Because of your awesome work I now know what is wrong with one of my plants, it is my first outdoor grow. Holes in the ground with bagged soil. That weird leaf pattern/ discoloration with splotchy yellow green showed up on one of the plants and I had no clue what kind of nutrient deficiency it was. Growth also stunted. Tried all kinds of methods to correct it the plant did not respond. None of my growing friends had a clue and now I know why. I am 3 weeks into flower I have 2 plants right next to it, that seem to be healthy and doing fine or should I say less affected, while the third is infected as I mentioned. I am torn if I should rip the third one out of the ground or let it run its course. It has been in close proximity to the other 2 for the entire grow. Thank you again and of course any insight is appreciated.
What about when it persist in the meristem. Like the purple phytoplasma? TC seems obsolete in that scenario.
Also, what about the slight mutations that happen via TC when not fed the appropriate catalyst.