DIY Tissue Culture: How to Get Started for Less Than $200
DIY Tissue Culture: How to Get Started for Less Than $200
Where I buy tissue culture supplies: https://www.plantcelltechnology.com/?ref=PLANTSINJARS
Find me on Instagram: https://www.instagram.com/plantsinjars_tissueculture/
Supplies List Below!
• Scale (0.01g accuracy): https://amzn.to/3I9fDRM
• pH Meter (0.01 pH accuracy): https://amzn.to/41CqJFA
• pH Up & Down: https://amzn.to/3pAM19r
• Spray Bottle: https://amzn.to/3pI3Q6g
• Plastic Gloves: https://amzn.to/41CBBmW
• Forceps: https://amzn.to/42K3VFa
• Scissors: https://amzn.to/3I9dwgK
• Sterile Petri Dishes: https://amzn.to/3nPNHLT
• mL Droppers: https://amzn.to/44X8cXl
• Agar: https://bit.ly/3o4yPcu
• Murashige & Skoog: https://bit.ly/3pMpAOr
• Kinetin: https://bit.ly/41IpXaa
• Polypropylene Containers: https://bit.ly/3pJrlMF
• Light Meter: https://amzn.to/42B1THI
Use code PLANTSINJARS for 10% off your Plant Cell Technology order.
Protocol for video: https://www.researchgate.net/publication/257148024_Micropropagation_of_self-heading_Philodendron_via_direct_shoot_regeneration
(I am aware that Philodendron Mamei is not technically a “self heading philodendron”, but this protocol has worked well for me before for other similar philos!)
Timestamps:
• 1:31 Building a still air box
• 3:18 Tissue culture supplies
• 4:45 What is a tissue culture protocol?
• 5:03 Where to find tissue culture protocols
• 5:44 What is tissue culture media?
• 8:55 How to read a tissue culture protocol
• 11:00 Making tissue culture media
• 13:52 Sterilizing the media
• 17:19 Prepping the still air box
• 18:30 Preparing the explants (tissue samples)
• 19:39 Putting the explant into tissue culture
• 25:30 Conditions for growth
• 26:19 When to subculture
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
I’m not interested in tissue culture (yet), but I want to try starting orchids from seed and this is a great starting point for handling sterile media and seeds outside of a laboratory
So how long does it take for the tissue to form into a plant with roots? Comparing this to Cloning.
Can you make a video for seaweed culture… as i am unable to make semi liquid media for seaweed culture…
I’m literally a dropout but I’m wanting to have my own farm one day so this’ll come in handy.
I need to try these with papaya
I’d have to buy a microwave though 🤦
Excellent tutorial!
🍾
I just found this video and at first I thought you were going to show ppl how to grow tissues..like the sneezing and blowing your nose type. I like plants but what’s the purpose of tissue planting? Is it the fun of cloning the plant or do you do something with them afterwards? Just an honest friendly question 🙂
Great vid! So much information. Thank you!
You r so sweet 😊 I home you doing well.
Spirit….🎉👍🙏🌏🌱
I’m desperate to get my hand on a cloudberry plant to do this but haven’t been able to find one rubus chamaemorus
Thanks perfect video!!!
God Bless You!!
Avocados
Thank you, your attention to detail & simplified short cuts are truly inspiring along with your enthusiasm in doing something you enjoy,
I grow a huge amount of perennial & annual garden plants & this year I bought in some tissue cultured delphinium & my knowledge in this is..& was limited but truly find your explanation a joy & considering setting up some basic laboratory myself. I currently have a ever growing collection let alone the ones I sell, it’s such fun & I have so many happy plants & ever more happy customers… 11 years in & so different from my training in carpentry..
Keep up the good work & love your posts, thank you 🌻
This tutorial was amazing!! I just had one question, how do you clean your equipment after using the PGR’s? I noticed you said never put any of the waste down the drain, so how do you clean your large glass container where you mixed the media? Thank you!!
could you advise how many mg of pure 99% Kinetin powder to 500ml water?
Hey do you have any recommendations for date palm tissue culture?
Hello, Can I ask you if there is a good alternative for murashige and skoog that is affordable for high school students like me? Coz most of the Murashige and Skoog I found online cost a lot more than my group’s budget and we really are having a hard time to find an affordable one
Could you make a video of making Keiki paste using BAP6 (benzylamino purine) , and GA3 please. I want to do it to generate tons of buds in my cacti. 🌵
Would these steps and ingredients apply for cannabis?
Can we use this method for plant with wood, as example citrus, mango, etc
I bought that exact same scale from amazon, i returned it because it did not register 300 milligrams at all. The only reason i knew that weight was because a friend gave me hers.
🤓
Damn girl, this is exactly what I need right now, a mix of my mycology stuff and plants 😎
WOW. Great video thanks! Interested in mycology and building a laminar flow hood..this will be a brilliant skill for my food forest propagation. Nice of you to share this information.
what about GA3 is it also unsafe like BAP?
How long can plants be stored in tissue culture without sub-culturing or propagating? My 30 year old Master Kush clone needs a new take on life.
Awesome video. Is it possible to clone palm trees using this technique?
Can I have the list of all inredoents or chemicals use in tissue culture?
I am a hobbyist plant breeder. What I really would like to be able to do is embryo and ovule culture. Finding your channel has me thinking it could be in the realm of possibility.
Can I do mushroom tissue culture following this technology?
This video was very well put together. Thank you!
Look like you make more plastics than you have plants…..not very green 😢
Microbiologist here. fun fact, when you use anything that does not reach autoclavable temperature, you will be selecting for spore forming microbes of the bacillus genus. The spores can survive extremes temperatures and can be very nasty and make you sick.
I smell underground mycology… I like it
I do not recommend cloning plants. In the wrong environment and in the wrong circumstances like by or around the wrong people natural order finds if disrespectful and offensive. I do not think you are capable of making those decisions. I am, but others are not. 🖕
Absolutely awesome!! You are an inspiration to amature botanists like me who always wished but missed the courage to start such a project. At 56 you have inspired me to start tissue culture for my regenerative farming particularly medicinal plants projet in India.
I heard that u talked about auxine but i dont see u use it in the video ?? Is it necessary ?
Poor thing, i hope my hand doesn’t get hurt doing tissue culture. Great intro video about this topic. New subscriber here 😎
Did you try to clone your right hand?
AWSOME….thank you very much ,,it was really helpful..
I’m glad I stopped by and checked it out..👏
Thank you so much for an informative video
Do you have a link to the lids and type of jars you use? Thank you!
SO Cool ty for the info!
Thanks for this awesome work you’ve put together. Sorry you got injured. For anyone who will be using scalpels, I highly recommend using a needle driver to place and remove the scalpel from the handle.
Honey works perfectly
Woa, this is it!!! ,thakyou for the instruction 😮 👍👍👍