How to Build a Home Tissue Culture Laboratory
How to Build a Home Tissue Culture Laboratory
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Lab Equipment and Supplies:
○ PCT Laminar Flow Hood https://rebrand.ly/ho9drgu
○ Magnetic Stirrer https://amzn.to/477dSP0
○ Scale https://amzn.to/49kTnQB
○ Glass Bead Sterilizer https://amzn.to/3saaHY7
○ Lab Glass Bead Sterilizer https://amzn.to/3SnVkpq
○ Alcohol Lamp https://amzn.to/3FEphdv
○ BactiZapper https://www.benchmarkscientific.com/product/b1000/
○ 1L Glass Containers https://amzn.to/47gRAKF
○ Wide mouth 1L Glass Containers https://amzn.to/3tRXZxo
○ Disposable Pipettes https://amzn.to/3tRXymK
○ 1 mL – 10 mL Pipettor https://amzn.to/47hgYA8
○ 1 mL – 10 mL Pipette Tips https://amzn.to/40kSIdY
○ 100uL – 1000uL Pipettor https://amzn.to/3tYz3Ez
○ 100uL – 1000uL Pipette Tips https://amzn.to/49hxrWJ
○ Pour Boats https://amzn.to/3Mq3UQR
○ Pressure Cooker https://amzn.to/3QFlumr
○ MS Basal Medium https://rb.gy/2g3zww
○ Gellan Gum https://rebrand.ly/nh51u7h
○ Agar https://rebrand.ly/ks8n2hx
○ BAP https://rebrand.ly/jwpoouu
○ NAA https://rebrand.ly/olpm030
○ IBA https://rebrand.ly/xonzm20
○ Kinetin https://rebrand.ly/y8jkm9c
○ pH Meter https://amzn.to/3MvUu6y
○ KCl solution https://amzn.to/3SxKQ71
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○ Forceps https://amzn.to/4622YJr
○ Scissors https://amzn.to/47mjm8u
○ Strainer https://amzn.to/3SwiF8u
○ Surgical Tray https://amzn.to/45Vz4Xa
○ Reusable Containers https://rb.gy/0mb0ql
○ Reusable Magenta Boxes https://rb.gy/qoti8h
○ Grafting Tape (for sealing jars) https://amzn.to/3Sqs41h
○ Shelves https://amzn.to/46WIoeE
○ Hortipower Lights https://www.hortipower.com/products/hortipower-city-farming-linear-20w-1200mm-ac-input
○ Cheaper Lights that also work https://amzn.to/3QJ3Jm6
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
2:57 hate it when that happens 😉
Great intro to getting started, 👏👏👏
0:59 yay weebs!
Hello we want tissue culture dates plant please help me
I subscribe your channel
you know what would be cool? a tissue culture terrarium.
specifically designed to use tissue culture techniques for aesthetic purposes, not propagation. with no soil or anything boring like that.
like a nutrient solution aquarium with tons of terrestrial plants in it or like a tube filled with plant balls.
I think these sterile plants floating in tubes with nutrient solution just look cool af.
or like little self contained plant jars with LEDs that you can put in dark rooms for decoration.
and making it look like interior design of the future 😂
perhaps you could even sell them🤑
What volume range should I be looking at to buy a pipette?
How long do the HEPA filters last on your cabinet?
Love the sense of mild shame/self recrimination you have over owning not two, but four pressure cookers 🤣
Can you do a video that is the whole process from start to finish (initiation to acclimating)? I know it would take months to be ready, but it would be interesting to see the progress with date stamps, etc.
Keep your blue lab in water or storage solution at all times. Submerged just past probe
Ouuuu *bonk
I saw some people say you can us an insta pot for took autoclaving
Вот бы на русском
I’m a home mycologist, started in a carpet room with no SAB or flow hood and yes, I’ve set gloves on fire while culturing more than once, I just don’t use gloves no more, and I don’t have carpet now 🎉
Venusflytrap please. Tenks
you need 70% alcohol to sterilize because that is the proper percentage to effectively lyse the cell wall of bacteria and sterilize a surface area. It has nothing to do with evaporation.
Can I use petri dishes for culture vs. the plastic containers you use I have boxes of glass petri dishes? I’m wondering if there is enough room in the dishes.
Hi you are amazing, please make video on Banana tissue culture and Mango. Need step wise tissue culture video for these plants
Your editing is perfect!! Constant knowledge and keeps you interested.
Really funny. Wasn’t expecting that lol
For lights I highly recommend the YouTube channel MIGRO. Shane does Phenomenal work and breaks down all of the finer details for how plants react to light and the photon efficiency as well as the total equipment efficacy.
Can you make a plant from a piece of grapes from the store? Or only from the leaves?
Hi I feel lucky to find your channel I have some questions
you are so amazinggg 🙂
Thank you so much for the video!
Enjoying the videos! Curious if you (or anyone else reading this), knows where to get the supplies related to plant cell tech, in Europe? As paying over 100 dollars for shipping is not ideal. Thanks!
I look through your videos and your content really inspires me and everything looks pretty straightforward, but some of my friends keep telling the way you introduce the whole process of TC is "just for fun" and they mainly point out that having a "DIY" laminar cabinet will drastically lower the overall results in TC. I as a person who’s just interested in this sphere, will definitelly have extremely miserable results. Could you please tell if having a "professional" laminar cabinet/autoclave increase the chances? If so how much? Will it require the person to go deeper and deeper into this area to get some visible results? Cause it feels like without having more profound knowledge about this issue, it will waste time and money. I understand that you have really big experience of many years studying and researching and doing TC, but hand on heart will the person "who just started" have sooner results or not, especially having a DIY tools? Thanks)
pressure cooked paper towels?
Can you recommend particular devices required to start Home Tissue Culturing Indoor & Outdoor Exotic plants.
I think, i love that girl
Hi, I’m Keith and I have started an alcohol fire.
Really glad you said you are a menace to probes lol
Also guilty, so made me feel better
What can be used to lift your racks in the PC so high?
Ty for all the great tips. $50 000 sounds like a very expensive scalpel.
Could you use a UV sterilizer? One designed for phones and toothbrushes and stuff?
In the cultivation of mushrooms, the community successfully sterilizes @ 12psi for 45 minutes for agar. I’m guessing it would also work here.
Love your stuff and your humor, keep it coming!
This was very helpful. Thank you so much.
I’m loving your channel, thanks.
It would be good to hear what your lab’s “business model” is.
How do you go about choosing plants to propagate and selling them commercially?
I assume that this isn’t just a serious hobby!
If you are taking a tissue culture from a plant and your placing it in a soy protein that was genetically modified, does that make the plant genetically modified as well when the new plants are planted.
I know that’s a stupid question but I don’t believe in stupid questions so there you go..
$50k medical bill for cutting a tendon?!?! Wow! Thank God I live in Australia 🇦🇺
You are flowing source of knowledge, THANK YOU!!
What is the largest size glass container Theoretically a tissue culture plant can be kept in? Is there limitations to size? Do you think a one gallon jar would be considered unreasonable? I want the plant to be able to get 8"-10" tall, That way I don’t have to mess with them as much…
I couldn’t find in your videos what kind of paper you use to make the micropropagation in the sterile cabinet. do you use normal kitchen paper or another kind? do you sterilize the paper in the autoclave? how is it done? thank you very much for your videos and your work!
I thought pressure cooking psi determines where you live? You state 15 psi but what state do you live in?
Fun fact: all of this gear is also appropriate for mycologists
3:21 English isn’t my native language, and I didn’t get what she said about ‘landlord’ or something like that. Probably, is just a joke (I noticed her humor sense hehe), but I’m curious about this. Can someone explain it to me?
You killed me “@hello gamers!”😂. I feel exposed! 🤣
Do you have any recommendations on a specific desk to buy? The only one I have right now is from Ikea and it has grooves on it which makes it impossible to sterilize.